Site-directed Mutagenesis

Sodium-Dependent Neutral Amino Acid Transporter Type 1 Is an Auxiliary Receptor for Baboon Endogenous Retrovirus

Kinetics / Virology / Biological Chemistry / Endogenous Retroviruses / Gene expression / Cats / Biological Sciences / Papio / Cell line / Humans / Sequence alignment / Liver / Mice / Animals / T cells / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Rats / Adult / Glutamine / Sodium / HeLa cells / Amino Acid Profile / Cysteine / Alanine / Host Range / Amino Acid Sequence / Base Sequence / Cell Surface Markers / Site-directed Mutagenesis / Histidine / cDNA library / Oligosaccharides / Immunoblotting / Molecular Sequence Data / Cell Membrane / Cats / Biological Sciences / Papio / Cell line / Humans / Sequence alignment / Liver / Mice / Animals / T cells / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Rats / Adult / Glutamine / Sodium / HeLa cells / Amino Acid Profile / Cysteine / Alanine / Host Range / Amino Acid Sequence / Base Sequence / Cell Surface Markers / Site-directed Mutagenesis / Histidine / cDNA library / Oligosaccharides / Immunoblotting / Molecular Sequence Data / Cell Membrane

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Familial Porphyria Cutanea Tarda: Characterization of Seven Novel Uroporphyrinogen Decarboxylase Mutations and Frequency of Common Hemochromatosis Alleles

Argentina / Heterogeneity / Biological Sciences / Genetic counseling / Humans / Mutation / Polymerase Chain Reaction / Exploration / Pigments / Pigment / Photosensitivity / Long Range / Genetic determinism / Skin / Gene / Phenotype / Hemochromatosis / Introns / Risk Factor / Gen / Genotype / Uroporphyrinogen Decarboxylase / Base Sequence / Amino Acid Substitution Rates / Recombinant Proteins / Site-directed Mutagenesis / Haplotype / The American / Molecular Sequence Data / Mutation / Polymerase Chain Reaction / Exploration / Pigments / Pigment / Photosensitivity / Long Range / Genetic determinism / Skin / Gene / Phenotype / Hemochromatosis / Introns / Risk Factor / Gen / Genotype / Uroporphyrinogen Decarboxylase / Base Sequence / Amino Acid Substitution Rates / Recombinant Proteins / Site-directed Mutagenesis / Haplotype / The American / Molecular Sequence Data

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Familial Porphyria Cutanea Tarda: Characterization of Seven Novel Uroporphyrinogen Decarboxylase Mutations and Frequency of Common Hemochromatosis Alleles

Argentina / Heterogeneity / Biological Sciences / Genetic counseling / Humans / Mutation / Polymerase Chain Reaction / Exploration / Pigments / Pigment / Photosensitivity / Long Range / Genetic determinism / Skin / Gene / Phenotype / Hemochromatosis / Introns / Risk Factor / Gen / Genotype / Uroporphyrinogen Decarboxylase / Base Sequence / Amino Acid Substitution Rates / Recombinant Proteins / Site-directed Mutagenesis / Haplotype / The American / Molecular Sequence Data / Mutation / Polymerase Chain Reaction / Exploration / Pigments / Pigment / Photosensitivity / Long Range / Genetic determinism / Skin / Gene / Phenotype / Hemochromatosis / Introns / Risk Factor / Gen / Genotype / Uroporphyrinogen Decarboxylase / Base Sequence / Amino Acid Substitution Rates / Recombinant Proteins / Site-directed Mutagenesis / Haplotype / The American / Molecular Sequence Data

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Biochemical characterization of recombinant serotonin N-acetyltransferase

Engineering / Biochemistry / Thermodynamics / Technology / Catalysis / Kinetics / Virology / Calcium / Biological Chemistry / HIV / Aspergillus / Biological Sciences / Saccharomyces cerevisiae / PCR / Ubiquitin / Plant Genome Project / Tuberculosis / Humans / Sequence alignment / Mycobacterium tuberculosis / Escherichia coli / Mice / CPM / Female / Animals / Male / Glutathione / Physical sciences / Candida albicans / Phosphorylation / Proteins / Capsid / Affinity chromatography / SALT TOLERANCE / Enzyme / Casein Kinase 2 / Cattle / Molecular cloning / Rats / HeLa cells / Substrate Specificity / Pineal / Protein Conformation / Protein Kinase / Amino Acid Sequence / Base Sequence / Ribonucleoproteins / Amino Acid Substitution Rates / Recombinant Protein / Recombinant Proteins / Biological Macromolecules / Hydrogen-Ion Concentration / Asparagine / Fusion Protein / DNA binding proteins / Site-directed Mutagenesis / Sodium Chloride / Biochemistry and cell biology / Dimerization / Okadaic acid / Molecular Sequence Data / DNA mutational analysis / Kinetics / Virology / Calcium / Biological Chemistry / HIV / Aspergillus / Biological Sciences / Saccharomyces cerevisiae / PCR / Ubiquitin / Plant Genome Project / Tuberculosis / Humans / Sequence alignment / Mycobacterium tuberculosis / Escherichia coli / Mice / CPM / Female / Animals / Male / Glutathione / Physical sciences / Candida albicans / Phosphorylation / Proteins / Capsid / Affinity chromatography / SALT TOLERANCE / Enzyme / Casein Kinase 2 / Cattle / Molecular cloning / Rats / HeLa cells / Substrate Specificity / Pineal / Protein Conformation / Protein Kinase / Amino Acid Sequence / Base Sequence / Ribonucleoproteins / Amino Acid Substitution Rates / Recombinant Protein / Recombinant Proteins / Biological Macromolecules / Hydrogen-Ion Concentration / Asparagine / Fusion Protein / DNA binding proteins / Site-directed Mutagenesis / Sodium Chloride / Biochemistry and cell biology / Dimerization / Okadaic acid / Molecular Sequence Data / DNA mutational analysis

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Identification of amino acid residues critical for endonuclease and integration activities of HIV-1 IN protein in Vitro

Bacteriology / Catalysis / Flow Cytometry / Virology / Calcium / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data

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Identification of amino acid residues critical for endonuclease and integration activities of HIV1 IN protein in Vitro

Bacteriology / Catalysis / Flow Cytometry / Virology / Calcium / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Biological / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data / Biological Chemistry / Magnetic Resonance Spectroscopy / Gene expression / Cell separation / Biocatalysis / Signal Transduction / HIV / Biological Sciences / Cell line / Identification / Humans / Mutagenesis / Sequence alignment / Mutation / Placenta / Virus / Escherichia coli / Animals / Biological / Plants / Monoclonal Antibodies / Chemotaxis / Phosphorylation / Plasmids / Affinity chromatography / Phenotype / Phosphotyrosine / CHEMICAL SCIENCES / Molecular cloning / Amino Acids / Mycoplasma Genitalium / Lysine / Amino Acid Profile / Transfection / Protein Conformation / Amino Acid Sequence / Base Sequence / Amino Acid Substitution Rates / Chemokines / Structure activity Relationship / Newcastle Disease Virus / Recombinant Proteins / Protein Binding / Hydrolysis / DNA binding proteins / Site-directed Mutagenesis / Interleukin / Interferon Alpha / Down-Regulation / Molecular Sequence Data

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Intracellular Long-Chain Acyl CoAs Activate TRPV1 Channels

Phospholipids / Calcium / Multidisciplinary / Patch-clamp and imaging techniques / Lipids / Humans / Fatty acids / Mutation / PLoS one / Protein Secondary Structure Prediction / Amino Acid Sequence / Protein Binding / Site-directed Mutagenesis / Molecular Sequence Data / Humans / Fatty acids / Mutation / PLoS one / Protein Secondary Structure Prediction / Amino Acid Sequence / Protein Binding / Site-directed Mutagenesis / Molecular Sequence Data

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Recycling of aromatic amino acids via TAT1 allows efflux of neutral amino acids via LAT2-4F2hc exchanger

Physiology / Fluorescence Microscopy / Mice / Female / Animals / P-glycoprotein / Aromatic amino acid / Medical Physiology / High Performance Liquid Chromatography / Physical Interaction / European / Xenopus laevis / Amino Acid Profile / Base Sequence / Oocytes / Recombinant Proteins / Proximal Tubule / Site-directed Mutagenesis / Similarity Function / Small Intestine / P-glycoprotein / Aromatic amino acid / Medical Physiology / High Performance Liquid Chromatography / Physical Interaction / European / Xenopus laevis / Amino Acid Profile / Base Sequence / Oocytes / Recombinant Proteins / Proximal Tubule / Site-directed Mutagenesis / Similarity Function / Small Intestine

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Mutagenesis of important amino acid reveals unconventional homologous internalization of β1-adrenergic receptor

Life Sciences / Western blotting / Internalization / Cell line / Humans / Mutation / cyclic AMP / D-Aspartic Acid / Lysine / Radioligand Assay / Amino Acid Profile / Transfection / Propranolol / Site-directed Mutagenesis / Ligands / Biochemistry and cell biology / Isoproterenol / Cell Membrane / Mutation / cyclic AMP / D-Aspartic Acid / Lysine / Radioligand Assay / Amino Acid Profile / Transfection / Propranolol / Site-directed Mutagenesis / Ligands / Biochemistry and cell biology / Isoproterenol / Cell Membrane

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Heterologous expression in Escherichia coli of Neurospora crassa neutral trehalase as an active enzyme

Gene expression / Multidisciplinary / Neurospora crassa / Biological Sciences / Vancomycin / Aquaporins (AQP) / Mutagenesis / Copper / Escherichia coli / Animals / Polymerase Chain Reaction / Temperature / Phosphorylation / Biochemical / Affinity chromatography / Enzyme / CHEMICAL SCIENCES / Cattle / Molecular cloning / Protein expression and purification / Fluorescent Antibody Technique / Anti-Bacterial Agents / Active site / Rats / Specific Activity / Time Factors / Filamentous fungi / Protein Expression / Metal ion / Amino Acid Sequence / Base Sequence / Recombinant Protein / Heterologous Expression / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Glycosyl Hydrolases / Biochemistry and cell biology / Molecular Sequence Data / Aquaporins (AQP) / Mutagenesis / Copper / Escherichia coli / Animals / Polymerase Chain Reaction / Temperature / Phosphorylation / Biochemical / Affinity chromatography / Enzyme / CHEMICAL SCIENCES / Cattle / Molecular cloning / Protein expression and purification / Fluorescent Antibody Technique / Anti-Bacterial Agents / Active site / Rats / Specific Activity / Time Factors / Filamentous fungi / Protein Expression / Metal ion / Amino Acid Sequence / Base Sequence / Recombinant Protein / Heterologous Expression / Hydrogen-Ion Concentration / Site-directed Mutagenesis / Glycosyl Hydrolases / Biochemistry and cell biology / Molecular Sequence Data

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Human DNMT2 methylates tRNAAsp molecules using a DNA methyltransferase-like catalytic mechanism

RNA / Drosophila melanogaster / Catalytic Mechanism / Humans / Sequence alignment / Animals / Dictyostelium / RNA isolation / Enzyme / Amino Acid Sequence / Knock out / Site-directed Mutagenesis / Catalytic Activity / Biochemistry and cell biology / Molecular Sequence Data / Animals / Dictyostelium / RNA isolation / Enzyme / Amino Acid Sequence / Knock out / Site-directed Mutagenesis / Catalytic Activity / Biochemistry and cell biology / Molecular Sequence Data

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The human Dnmt2 has residual DNA-(cytosine-C5) methyltransferase activity

Biological Chemistry / Biological Sciences / Humans / Mice / Animals / DNA methylation / High Pressure Liquid Chromatography / CHEMICAL SCIENCES / Molecular cloning / Quntitative Thin Layer Chromatography / Site-directed Mutagenesis / DNA methylation / High Pressure Liquid Chromatography / CHEMICAL SCIENCES / Molecular cloning / Quntitative Thin Layer Chromatography / Site-directed Mutagenesis

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Poplar Peroxiredoxin Q. A Thioredoxin-Linked Chloroplast Antioxidant Functional in Pathogen Defense

Gene expression / Biological Sciences / Antioxidants / Populus / Plant Physiology / Plant diseases / Plant / Electron Transport / Thioredoxin / Peroxidases / Substrate Specificity / Amino Acid Sequence / Base Sequence / Recombinant Proteins / PLANT PROTEINS / Site-directed Mutagenesis / Oxidation-Reduction / Dimerization / Chloroplasts / Peroxiredoxins / Molecular Sequence Data / Plant diseases / Plant / Electron Transport / Thioredoxin / Peroxidases / Substrate Specificity / Amino Acid Sequence / Base Sequence / Recombinant Proteins / PLANT PROTEINS / Site-directed Mutagenesis / Oxidation-Reduction / Dimerization / Chloroplasts / Peroxiredoxins / Molecular Sequence Data

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Functional Characterization of Gne (UDP-N-Acetylglucosamine- 4-Epimerase), Wzz (Chain Length Determinant), and Wzy (O-Antigen Polymerase) of Yersinia enterocolitica Serotype O:8

Genetics / Bacteriology / Biological Sciences / Lipopolysaccharide / Crystal structure / Escherichia coli / Enzyme / Gram-negative bacteria / Lipopolysaccharides / Active site / Site-directed Mutagenesis / Yersinia Enterocolitica / Escherichia coli / Enzyme / Gram-negative bacteria / Lipopolysaccharides / Active site / Site-directed Mutagenesis / Yersinia Enterocolitica

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Combinatorial Engineering of Dextransucrase Specificity

Catalysis / Magnetic Resonance Spectroscopy / Multidisciplinary / Protein Engineering / Mutation / Glucans / PLoS one / Substrate Specificity / Glycosyltransferases / Amino Acid Sequence / Structure activity Relationship / Site-directed Mutagenesis / Leuconostoc / Glucans / PLoS one / Substrate Specificity / Glycosyltransferases / Amino Acid Sequence / Structure activity Relationship / Site-directed Mutagenesis / Leuconostoc

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Site-specific incorporation of non-natural residues into peptides: Effect of residue structure on suppression and translation efficiencies

Biochemistry / Organic Chemistry / Beta decay / Animals / Context Effect / Protein structure / Tetrahedron / Amino Acid Profile / Acylation / Rabbits / Amino Acid Sequence / Base Sequence / Chemical Synthesis / Site-directed Mutagenesis / Organic Compound / Nucleic Acid Conformation / Protein Biosynthesis / Biochemistry and cell biology / Organic Acid / Nucleic Acid / Protein structure / Tetrahedron / Amino Acid Profile / Acylation / Rabbits / Amino Acid Sequence / Base Sequence / Chemical Synthesis / Site-directed Mutagenesis / Organic Compound / Nucleic Acid Conformation / Protein Biosynthesis / Biochemistry and cell biology / Organic Acid / Nucleic Acid

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